Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
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چکیده
منابع مشابه
Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding w...
متن کاملCalcium-binding protein of the chick chorioallantoic membrane. II. Vitamin K-dependent expression
A simple method was devised for the maintenance of the chorioallantoic membrane (CAM) of chick embryos in organ culture. Explants of CAM survived for up to 5 days in this system and retained the characteristic three-layered morphology (ectoderm, mesoderm, and endoderm). Induction of the CAM calcium-binding protein (CaBP) by effectors of calcium metabolism was studied in these organ cultures. Vi...
متن کاملI . Immunohistochemical Localization CHICK ROCKY
The preparat ion of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioaUantoic membrane ( C A M ) is described. The antiC a B P appeared to be specific for the C a B P by immunodiffusion and immunoelectrophoresis. Applicat ion of the ant i -CaBP in immunofluorescence histochemistry revealed that the C a B P is present in the C A M only at developmental age...
متن کاملTransepithelial calcium transport in the chick chorioallantoic membrane. I. Isolation and characterization of chorionic ectoderm cells.
The chicken eggshell supplies approximately 80% of the calcium found in the hatchling chick. The mobilization of eggshell calcium into the developing embryo involves the transepithelial transport of large amounts of calcium in a development-specific manner. The cells responsible for the transport of eggshell calcium into the embryonic circulation are the ectodermal cells of the chorioallantoic ...
متن کاملCalcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization.
During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1978
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.77.3.743